Remediation biotechnology
Canada-based Adventus Group’s patented bioremediation process has found application in cleaning mineral oil (TPH) and petroleum volatile organic compounds (PVOCs) in the Netherlands. The proprietary EHC-OTM technology was selected by a consortium of Dutch remediation engineering and consulting companies for use at two industrial sites for cleaning up PVOC and TPH contamination caused by underground storage tanks leaking into soil and groundwater. The remedial action at these sites involved in situ bioremediation facilitated by the use of EHC-O, an oxygen-releasing compound that accelerates the aerobic biodegradation of certain organic constituents.


EHC-O is an integrated source of slow-release oxygen, major-, minor- and micro-nutrients, and a pH buffering agent. This unique combination of materials facilitates aerobic bioremediation of groundwater, sediment or soil environments impacted by various organic/inorganic compounds. For organic constituents amenable to aerobic biodegradation processes (for example, petroleum hydrocarbons, certain pesticides/herbicides), EHC-O significantly stimulates the catabolic activity of the indigenous microflora, thereby accelerating the removal rate of con- taminant. It is supplied in 77.175 kg pails as a powder that can be mixed with soil or slurried in water.


Installation techniques vary widely, depending on the application. For example, the powder can be mixed with soil and placed at the bottom of an excavated pit. A slurry can be made and the mixture injected into the subsurface using techniques like direct injection through Geoprobe rods or hydraulic fracturing. The powder is fine enough to allow injection of the slurry through well screens.


Website: www.solidwaste.com
Aerobic bioremediation for PAH-laden soil
Researchers at Gwangju Institute of Science and Technology in the Republic of Korea have studied the applicability of a combined process of solubilization and biodegradation using soil-packed column. In the solubilization step, 50 pore volumes of 150 mg/l biosurfactants solution was injected. The removal of phenanthrene was 17.3 per cent and 9.5 per cent from soil with pH 5 and 7, respectively. The highest solubility was detected at pH 5 and this result confirmed that adjusting the pH of the biosurfactants solution injected could help enhance the solubility of phenanthrene.
Following this, soil samples were completely transferred to batches and incubated for 10 weeks to monitor phenanthrene degradation. It was found that the phenanthrene concentration in all samples reduced significantly during the biodegradation step, except for the one that was flushed with biosurfactants solution at pH 4. This indicated that the degradation of contaminants by specific species might not be affected by the residual biosurfactants after application of the solubilization process. These results also suggested the possibility that the biosurfactant-enhanced flushing process could be developed as a useful technology without any negative effect on subsurface environments and could be combined with the biodegradation process to increase removal efficiency.


Contact: Mr. Shin K.H, Department of Environmental Science and Engineering, Gwangju Institute of Science & Technology 1, Oryong-dong, Buk-gu, Gwangju 500 712, Republic of Korea.


Website: www.ncbi.nlm.nih.gov
Harnessing bacteria to combat pollution
Researchers at the NRC Biotechnology Research Institute (NRC-BRI), Canada, report to have found a solution for cleaning up the millions of tonnes of cancer-causing chlorinated solvents that have been dumped, poured, buried or leaked into soil and water over the past few decades. NRC’s answer is simple: turn it into harmless carbon dioxide and water. The NRC-BRI process uses a proprietary two-in-one bioremediation technique that almost completely removes chlorinated solvents from the contaminated water. Presently, the bioremediation techniques available on the market use either aerobic or anaerobic bacteria, alone or consecutively. The NRC-BRI technology capitalizes on the insight that these bacteria can work together synergistically under just the right conditions.


Dr. Serge Guiot, Head of the NRC-BRI Environmental Bioengineering Group, said that the new method is based on the fact that aerobic and strict anaerobic micro-organisms can grow together in a single natural habitat such as, for instance, the biogranules. Biogranules are naturally occurring microscopic aggregations of bacteria that provide a natural tag-team environment for both aerobic and anaerobic bacteria. Aerobic bacteria live on the oxygen-rich surface of the granule, while anaerobic ones live in the oxygen-less core. This means that for bioremediation, the biogranules deliver a one-two punch and is the basis of this technology, notes Dr. Guiot.


Anaerobic bacteria begin the breakdown of chlorinated solvents while aerobic bacteria conclude the job, digesting the by-products of their cousins’ work. Carbon dioxide, water and harmless chloride salts are the only by-products of this tag-team digestion. The patented NRC tech-nology stimulates and accelerates this natural biodegradation by using electrolysis of water into oxygen and hydrogen to fuel the various bacteria. Anaerobic methanogenic bacteria use hydrogen to dechlorinate the solvents and produce methane. In turn, methane and oxygen energize the aerobic digestion of the end- products of anaerobic breakdown.


Website: www.nrc-cnrc.gc.ca
Treatment of sewage discharges

At the Australian Nuclear Science and Technology Organization, Dr. Tony Taylor has developed a technology for the purification of sewage and wastewater. The revolutionary membrane bioreactor cleanses the effluent streams to such an extent that the end product can be used for potable purposes. Dr. Taylor describes his invention as a simple arrangement of gills that uses bacteria to operate as a lung and a stomach. The system literally “eats” waste matter and “breathes” air, and so is self-perpetuating. The inexpensive nano-particulate membrane bioreactor (NMB) can be made in different sizes for houses, complexes or municipal treatment plants.


The secret of this technology is in the bioreactor’s unique membrane. “On one side of the membrane, cells are grown in direct contact with air and on the other side, the sewage and wastewater flows, but at the same time feeds the cells through the extremely porous material,” says Dr. Taylor. Compared with other membrane surface culture technologies, this process enables 50 times more biomass growth. “At about US$1/m2, this technology is cheaper than current membranes which can cost up to US$500/m2,” he said. There are many other uses to this invention, including antibiotic production, mining, bioremediation and aquaculture.


Contact: Australian Nuclear Science and Technology Organization (ANSTO), PMB 1, Menai, NSW 2234, Australia. Tel: +61 (2) 9717 3111; Fax: +61 (2) 95 43 5097


E-mail: enquiries@ansto.gov.au 


Website: www.azonano.com
Cleaning up soil pollutants
Biosaint Co. Ltd., the Republic of Korea, is offering a process for the rapid and highly efficient degradation of various soil pollutants. This process combines two technologies – washing to dissolve pollutants and bioremediation, in which selected micro-organisms are applied to the soil media. Important features of the process include:
 
  • Remediation to depths of 3-6 m possible;
     
  • Saturated soil or pollution under groundwater table can also be effectively treated; and
     
  • Land heavily polluted with hard-to-biodegrade pollutants can be ef-efectively cleaned through the use of Biosaint’s agent.
     

Contact: Biosaint Co. Ltd., 104-1, Sangdo-5 dong, Dongjak-gu, Seoul 156-746, Republic of Korea. Tel: +82 (31) 4785 271; Fax: +82 (31) 47 85 270


E-mail: info@biosaint.com


Website: www.apec-vc.or.kr

Removal of PAHs
Researchers at the National University of Singapore have developed a technology for the rapid clean-up of Polycyclic aromatic hydrocarbons (PAHs) from contaminated soil. The new method is based on a combination of non-ionic surfactant soil washing, followed by PAH removal from the wash water in a rotating biological contactor (RBC) reactor using immobilized Phanerochaete chrysosporium, the white rot fungus.


Experiments have shown that soil washing is able to remove over 90 per cent of PAH from the soil to the aqueous phase using only a low concentration of 0.5 per cent (w/v) Tween 80 surfactant with a soil/water ratio of 1:10 (w/v). Subsequent treatment of the PAH-contaminated wash water in the RBC reactor resulted in more than 90 per cent removal for any of nine PAHs.


The treatment has particular applications for: the rapid abiological remediation of soil for compliance with international environmental soil quality standards; and using white rot fungi to oxidize PAH in soil wash water for compliance with aqueous discharge guidelines.


Contact: Department of Chemical and Environmental Engineering, The National University of Singapore, #4 Engineering Drive, Singapore 117576. Tel: +65 6874 2186; Fax: +65 6779 1936.


Website: www.chbe.nus.edu.sg