Microbes help clean toxic waste dumps
Researchers from the Florida State University (FSU), the United States, are studying a bioremediation technology to clean up toxic waste dump sites. Toxic wastes from weapons manufacturing between 1951 and 1983 have been buried under 243 acres in an East Tennessee valley. These compounds have been leaching into groundwater, extending in radioactive plumes for miles from the contaminated site.


During the course of a forthcoming five-year study, FSU Associate Prof. Joel Kostka and his team will test the natural method which involves the stimulation of naturally occurring microbes to promote bacterial growth in the soil sub-surface that scrub it off potentially deadly radioactive metal. If the bioremediation process proves successful on the uranium, technetium, nitrates and other potentially lethal leftovers at the site, the process should work to mitigate contamination at more than 7,000 other sites nationwide and do so more economically and effectively than most conventional methods.


The FSU researchers and other research teams from several universities and national laboratories across the country are involved in the clean-up project. Together, these teams will develop models to help predict the rate at which levels of contamination drop when using natural and artificial remediation methods. Sub-surface changes would be closely monitored using geophysical methods that send acoustic, electric and other signals into the ground. Prof. Kostkas team will lead the subsurface microbiology portion of the project.


Website: www.joelkostka.net 


Website: www.sciencedaily.com 
Permeable reactive barrier technology
A former manufactured gas plant in Southwest England is the site of an operational gas distribution depot. Application of conventionally used remediation strategies was not feasible owing to constraints posed by structures above and below the surface. A permeable reactive barrier (PRB) specifically, a SEquential REactive BARrier (SEREBAR) was selected as the best remediation option for this site.


The SEREBAR is currently treating groundwater contaminated with cyanide, polycyclic aromatic hydrocarbons and petroleum hydrocarbons. The system comprises an impermeable bentonite slurry barrier 200 m long installed across active gas mains to intercept contaminated groundwater and direct it into the capture zone of the SEREBAR system. The groundwater flows into the system through an interceptor and six treatment canisters that remediate the contaminated water using sequential anaerobic and aerobic treatments populated by indigenous bacteria. A back-up system of granular activated carbon is provided. Monitoring of results since start-up show that the contaminants in the discharge are significantly lower than the negotiated clean-up levels.


Website: www.clu-in.org
Bioremediation for petroleum soil/water contamination
CW3M Co. Inc., the United States, has patented methods and compositions that facilitate site-specific bioremediation of groundwater and soil with petroleum contamination. In situ bioremediation involves injecting a biomass including micro-organisms and associated nutrients, minerals and co-solvents directly into the groundwater or soil contaminant plume in strategic locations based on geophysical site characteristics of the sites subsurface. As the biomass slurry comes in contact with the contaminant, degradation begins. The micro-organisms trans-form ingredients of the slurry, plus nutrients from the environment, into cell-building materials and extracellular by-products such as water and carbon dioxide.


The concentration of the individual indigenous micro-organism strains and their relative percentages vary with the concentration of the pollutants in the soil and groundwater. Also, the rate of biodegradation is proportional to the types and concentration of the bacteria available to do the work. Under normal conditions, the bacteria will double as frequently as every 15-20 min until they reach a population of several billion per gram of soil. This method is suitable for contamination resulting from kerosene, diesel oil, fuel oil or petrol, with the total petroleum hydrocarbons being below 50,000 ppm. The hydraulic conductivity of the site has to be greater than 10-7 cm/s.


Website: www.freepatentsonline.com
Fungal strain aids bioremediation
Researchers in Egypt have isolated a fungal strain that exhibits high affinity to adsorb and degrade crude petroleum oil. Nutritional and environmental factors that affect petroleum degradation were evaluated by applying Placketl-Burman design, where K2HPO4, inoculum size and pH were the most significant variables. To optimize the level of the significant factors, Box-Behnken design was carried out and a mathematical model created. Further, a maximum petroleum oil degradation of 98.8 per cent has been proved.


Contact: Mr. Gaballa/Mr. Hussein/Mr. Abdel-Fattah, Mubarak City for Scientific Research & Technology Applications, New Borg El-Arab City, Alexandria 21934, Egypt.


Website: www.wipo.int
Enhanced control of in situ bioremediation
Gas Technology Institute, the United States, has patented method for the removal of volatile and semi-volatile contaminants from groundwater. In this process, the contaminants are stripped from the groundwater by sparging with an inert carrier gas. The stripped contaminants are then transported to a bioactive zone into which at least one gaseous oxidant and one nutrient are independently injected, to stimulate at least one microbial culture associated with the bioactive zone to remove the stripped contaminants from the bioactive zone.


By decoupling the sparging of the groundwater from the introduction of oxidants and nutrient feed gases into the bioventing bioactive zone of the subsurface, the oxygen and nutrient inputs to the bioactive zone can be adjusted (or carburetted) and controlled independently from the hydrocarbon input to the bioactive zone to achieve good kinetic performance in the bioactive zone while avoiding the problems of plugging of groundwater sparging due to biofilm and precipitate formation.


Website: www.freepatentsonline.com
Vermiculture for eco-friendly remediation
Researchers at the Institute for Ecosystem Studies, Italy, have studied, in laboratory scale, the effects of some bioremediation treatments on polluted soil and the use of specific parameters to study the evolution of biochemical processes that take place during soil decontamination. The bioremediation treatments were: compost; compost plus earthworm, Eisenia fetida; and a cocktail of micro-organisms, enzymes and nu-trients. Soil without treatment was used as the control.


Chemical, physico-chemical, biolo-gical and biochemical parameters were determined to assess the efficiency of the processes involved in the degradation of hydrocarbons. Investigations revealed an intense microbiological activity expressed as carbon dioxide (CO2) generation. The two compost treatments had the highest CO2 release, indicating the availability of organic substrate characterizing the compost.


The organic substrate reduction decreased hydrolytic enzyme activities representative of carbon (-glucosidase), nitrogen (protease) and phosphorus (phosphatase) cycles. The enzyme activities showed their highest values in the treatments with compost. The greatest hydrocarbon reduction was found in the treatments with compost, particularly with earthworms.


Contact: Dr. Grazia Masciandaro, Institute for Ecosystem Studies, Soil Chemistry Research Unit, Via Moruzzi 1, 56124 Pisa, Italy. Tel: +39 (50) 3152 481; Fax: +39 (50) 3152473


E-mail: grazia.masciandaro@ise.cnr.it


Website: www.ingentaconnect.com
Bioremediation of water with chemical contamination
Shell Oil Co., the United States, has developed a process and apparatus for the in situ bioremediation of aquifers contaminated with chemicals, particularly oxygenated chemicals such as MTBE and t-butyl alcohol (TBA). The process involves injecting a microbial culture into the aquifers to degrade the said chemicals. The process uses:
 
  • A bacterial culture for the aerobic degradation of target chemicals
     
  • An apparatus for continuously or intermittently delivering bacterial culture to the sub-surface without backflow of soil and other materials; and
     
  • An oxygen delivery system that injects an oxygen-containing gas at a pressure of at least 5 psig above the hydrostatic pressure at each delivery point, by pulsed injection, at a frequency range of one per week to 10 per day.
     

Website: www.freepatentsonline.com

Rhizosphere remediation process
Researchers at Han-Yang University in the Republic of Korea have developed a process for treating soils contaminated with explosive materials like TNT or DNT. The low-cost and high-efficiency enhanced rhizosphere remediation technology is different from conventional processes such as phytoremediation.


Indigenous bacteria having the ability to decompose explosives were isolated and identified. By adding these to certain native plants, the team developed a biological treatment process that could effectively treat explosive-contaminated soil. It was found that micro-organisms KT22 exhibited the highest removal efficiency in TNT-contaminated soil, for both liquid and solid contaminants.


Contact: Hanyang University, Republic of Korea.


E-mail: w3master@ihanyang.ac.kr


Website: www.hanyang.ac.kr


Website: www.apec-vc.or.kr