Scientists uncover plants’ vitamin C secret
Agricultural scientists have uncovered the last big secret of vitamin C in plants. The breakthrough in understanding just how plants manufacture vitamin C will enable Hortresearch, New Zealand, to identify DNA markers for individual plants that naturally produce high levels of the vitamin. These plants are likely to be used in selective breeding programmes to produce fruits with more vitamin C in a form easily retained by the body, unlike large doses taken in vitamin pills.


Hortresearch’s General Manager (Science), Dr. Bruce Campbell, said his team has isolated the last undiscovered enzyme and proved that it controlled vitamin C in plants. The scientists studied kiwifruit, a plant naturally high in vitamin C, with typical green kiwifruit – bred from Actinidia deliciosa – containing about 100 mg in each 100 g of fruit. They also worked on an inedible wild kiwifruit variety called Actinidia eriantha – with a white, hairy skin, which is easy to peel – because it contains a massive 800 mg of vitamin C per 100 g.


Source: www.archives.foodsafety.ksu.edu 
Sorghum may benefit from cloned toxicity-tolerant gene
When soils are too acidic, the aluminium that is locked up in clay minerals dissolves into the soil as toxic ions, making it hard for most plants to grow. Aluminium toxicity in acidic soils limits crop production in as much as half the world’s arable land, mostly in developing countries in Africa, Asia and South America. Now, researchers at Cornell University, the United States, have cloned a new aluminium-tolerant gene in sorghum and expect to have novel genetically engineered aluminium-tolerant sorghum lines by next year. Sorghum is an important food crop in Africa, Central America and South Asia and is the world’s fifth most important cereal crop.


Research by Dr. Leon Kochian – a Cornell adjunct professor of plant biology and director of the Plant, Soil and Nutrition Laboratory of the United States Department of Agriculture Agriculture Research Service (USDA-ARS) – and colleagues showed that in aluminium-tolerant sorghum varieties, special proteins in the root tip release citric acid into the soil in response to aluminium exposure. Citric acid binds aluminium ions very effectively, preventing the toxic metal from entering the roots.


The researchers used genetic mapping to identify a single gene that encodes a novel membrane-transporter protein responsible for the citric acid release. They discovered that the gene is only turned on to express the protein and transport citric acid when aluminium ions are present in the surrounding soil. The researchers have now used the sorghum gene to engineer aluminium-tolerant transgenic Arabidopsis thaliana and wheat plants. The map-based cloning of this agronomically important gene in sorghum is helping advance this species as a model for further exploring the aluminium tolerance mechanisms and discovering new molecular genetic solutions to improving crop yields, Dr. Kochian said.


Source: www.news.cornell.edu 
Genetically modified eucalyptus as carbon sink
Eucalyptus trees genetically modified by a team of biologists from Taiwan and the United States have proven capable of ingesting up to three times more carbon dioxide (CO2) than normal strains, indicating a new path to reducing greenhouse gases and global warming. The scientists from the Taiwan Forestry Research Institute (TFRI) and North Carolina State University in the United States carried out the gene modification project that not only creates eucalyptus with a higher than normal CO2 absorptive capacity, but also causes them to produce less lignin and more cellulose.


TFRI researcher Dr. Chen Zenn-zong explained that cellulose, hemicellulose and lignin in trees are all created from carbon elements. “However, only cellulose can be used in commercial processes of pulp manufacture and bio-ethanol extraction,” he added. The project therefore aims to increase the value of genetically modified eucalyptus to related industries by adjusting the ratio of lignin and cellulose. “Meanwhile, we enhance the tree’s capacity in absorbing CO2 to reduce greenhouse gases, so that more trees planted for production, the more CO2 are consumed,” said Dr. Chen. With every eucalyptus carrying 18 per cent less lignin and 4.5 per cent more cellulose, he estimates that a pulp factory with an annual output of 1 million tonnes could generate extra revenue of about US$36 million every year.


Source: www.agbios.com
Discovery promises more nutritious cassava
Scientists from the International Centre for Tropical Agriculture (CIAT), Colombia, have developed a new cassava variety that might be more nutritious and easier to digest than other varieties. Cassava is the staple food for millions of poverty stricken people in Sub-Saharan Africa, South America and parts of Asia. Cassava root is rich in carbohydrates and starch, but low in protein and vitamins. Compared with other starchy crops, cassava contains relatively higher levels of amylose, which render it difficult to digest.


Dr. Hernan Ceballos and his colleagues from CIAT identified a new cassava variety with significantly reduced amylose content. Compared with traditional hard-to-digest cassava varieties with 17-25 per cent amylose content, the mutant contains an average of only 3.4 per cent amylose. The scientists found no reduction in its starch content; therefore it can provide more carbohydrates compared with traditional varieties. This is the first report of a natural mutation in cassava that resulted in drastic reduction of amylose content in root starch. Besides being more nutritious and easily digestible, the new variety may also be better suited for bioethanol production.


Source: www.isaaa.org
GM potatoes with better freezing tolerance
Potato, the fourth most important food crop, is a frost-sensitive species incapable of cold acclimation. A brief exposure to frost can significantly reduce yield, while hard frosts can completely destroy entire crops. Improvement in its freezing tolerance by just a few degrees would thus be of considerable benefit. A group of researchers from Oregon State University and Michigan State University in the United States, and Kyung Hee University in Republic of Korea, has genetically altered potato to be more tolerant to freezing.


Although genetic donors, particularly the cold-acclimatized wild potatoes of South America, exist, transfer of freezing tolerance to cultivated plants proved to be unsuccessful because of the complex genetics of the trait and the introduction of undesirable agronomic properties. By introducing the AtBCF genes for freeze tolerance from Arabidopsis with an activation control (promoter) only for cold conditions, scientists successfully obtained several potato lines with increased freezing tolerance of up to -5°C. They also found out that the attachment of the cold-inducible promoter minimize the expression of agronomically undesirable traits, like delayed flowering and retarded growth, previously attributed to the AtBCF genes.


Source: www.isaaa.org
New flood-tolerant rice variety
The Philippine Rice Research Institute (PhilRice) has developed a new rice variety tolerant to flood water and resistant to the bacterial blast, stemborer and tungro, three of the most dreaded rice pests.


The new variety, Tubigan 7, was developed using the DNA marker-assisted selection (MAS) technique. It is the first-ever successful DNA-MAS product developed in the Philippines, and the second locally developed biotech rice, after the tissue cultured variety of improved traditional Wagwag grains. Tubigan 7 has a fertility restorer trait and could yield about 8 tonnes per hectare during the dry season cropping and 5-6 tonnes per hectare during the wet season, and yields about 15 percent higher than the conventional harvest record in the Philippines.


Dr. Antonio A. Alfonso, head of plant breeding and biotechnology division of PhilRice, emphasized that Tubigan 7 is not a genetically modified crop, as the new variety was obtained through conventional breeding. The country’s National Seed Industry Council (NSIC) has officially released it as a variety.


Source: www.isaaa.org
New corn high in phytase
Chinese scientists have developed a genetically modified (GM) corn that could help improve the nutritional value of livestock feed and reduce pollution. The research work was carried out by the Chinese Academy of Agricultural Sciences (CAAS), and the corn has now entered pre-production field trials. The GM corn produces seeds containing high levels of the phytase enzyme, which helps livestock to digest phosphorus. Animals lack phytase in their system. As a result, farmers have to add the enzyme to animal feed to help livestock digest phosphorus.


The CAAS scientists isolated the gene that produces phytase from the fungus Aspergillus, and inserted it into corn. Initial tests have shown that the rate of seed germination, growth speed and yield of the GM corn are no different as compared with regular varieties. The scientists said that, under current industry criteria for feed additives, adding just a few grams of the GM corn seed per kilogram of animal feed would satisfy livestock’s nutritional demand for phosphorus. If the technology is commercialized, Chinese farmers could save up to US$60 million per year in buying industrial phytase.


Source: www.agbioworld.com
Marker-free GM Soybean produces by gene excision
Selectable marker genes are used in plant transformation systems to select transgenic events, but often the marker gene is no longer needed after the transgenic plants are regenerated. Their continued presence has always been the focus of criticisms in genetic improvement of crops. A group of researchers from DuPont recently produced transgenic soybean lines, which are free from marker genes and tolerant to glyphosate, through a self-activating gene excision system.


Unlike other approaches to produce marker-free plants, the gene excision system employed by the researchers delivers precise outcomes and does not require additional manipulations of the transformation and regeneration process. The glyphosate tolerance and marker genes were introduced along with a gene coding for the enzyme Cre recombinase, which will remove itself and the marker gene immediately upon induction. This self-activating gene excision strategy is currently being applied to numerous plants like maize, cotton and groundnut, and many coniferous trees.


Source: www.isaaa.org